The Histopathology Core provides technical histology and interpretive pathology services to support IMS researchers and the greater scientific community.

---

Services offered by the Histopathology Core:

Routine histology techniques include processing, embedding, and sectioning of fixed and frozen samples. Staining options range from Haematoxylin and Eosin (H&E) to special stains such as Picrosirius Red and Masson’s Trichrome.

The Core uses a Leica Bond RX autostainer to run high-throughput single and multiplex immunohistochemistry (IHC) and immunofluorescence (IF) assays. Automated in situ hybridisation (ISH) is available through RNAscope by ACD Bio. RNAscope uses a proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridisation. The assay can label up to four RNA targets in a single section and can combine with IHC to demonstrate protein/RNA co-localisation in a single section.

Histopathology offers one-to-one sessions with a pathologist for slide reviews and training. Our pathologists can also provide quantitative analysis reports and images for publication. The Core can access additional support from pathologists with various specialities where required.

---

Facilities and equipment available:

• Sakura VIP6 Enclosed Tissue Processor
• Leica Bond RX Autostainer
• Thermo/Epredia HistoStar Embedding Centre
• Leica RM2255 Rotary Microtome
• Leica CM1950 Cryostat

---

Recent publications involving the Histopathology Core:

IMS

• Mann JP, Duan X, Patel S, Tábara LC, Scurria F, Alvarez-Guaita A, Haider A, Luijten I, Page M, Protasoni M, Lim K, Virtue S, O’Rahilly S, Armstrong M, Prudent J, Semple RK, Savage DB. A mouse model of human mitofusin 2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion. Elife. 2023 Feb 1;12:e82283. doi: 10.7554/eLife.82283.
• Patel S, Haider A, Alvarez-Guaita A, Bidault G, El-Sayed Moustafa JS, Guiu-Jurado E, Tadross JA, Warner J, Harrison J, Virtue S, Scurria F, Zvetkova I, Blüher M, Small KS, O’Rahilly S, Savage DB. Combined genetic deletion of GDF15 and FGF21 has modest effects on body weight, hepatic steatosis and insulin resistance in high fat fed mice. Mol Metab. 2022 Nov;65:101589. doi: 10.1016/j.molmet.2022.101589.
• Schoonejans JM, Blackmore HL, Ashmore TJ, Pantaleão LC, Pellegrini Pisani L, Dearden L, Tadross JA, Aiken CE, Fernandez-Twinn DS, Ozanne SE. Sex-specific effects of maternal metformin intervention during glucose-intolerant obese pregnancy on body composition and metabolic health in aged mouse offspring. Diabetologia. 2022 Sep 16. doi: 10.1007/s00125-022-05789-0.
• Pellegrinelli V, Rodriguez-Cuenca S, Rouault C, Figueroa-Juarez E, Schilbert H, Virtue S, Moreno-Navarrete JM, Bidault G, Vázquez-Borrego MC, Dias AR, Pucker B, Dale M, Campbell M, Carobbio S, Lin YH, Vacca M, Aron-Wisnewsky J, Mora S, Masiero MM, Emmanouilidou A, Mukhopadhyay S, Dougan G, den Hoed M, Loos RJF, Fernández-Real JM, Chiarugi D, Clément K, Vidal-Puig A. Dysregulation of macrophage PEPD in obesity determines adipose tissue fibro-inflammation and insulin resistance.  Nat Metab. 2022 Apr;4(4):476-494. doi: 10.1038/s42255-022-00561-5.
• Cacciottolo TM, Henning E, Keogh JM, Bel Lassen P, Lawler K, Bounds R, Ahmed R, Perdikari A, Mendes de Oliveira E, Smith M, Godfrey EM, Johnson E, Hodson L, Clément K, van der Klaauw AA, Farooqi IS. Obesity due to Steroid Receptor Coactivator-1 deficiency is associated with endocrine and metabolic abnormalities. J Clin Endocrinol Metab. 2022 Feb 8:dgac067. doi: 10.1210/clinem/dgac067.

EXTERNAL

• Rahrmann EP, Shorthouse D, Jassim A, Hu LP, Ortiz M, Mahler-Araujo B, Vogel P, Paez-Ribes M, Fatemi A, Hannon GJ, Iyer R, Blundon JA, Lourenço FC, Kay J, Nazarian RM, Hall BA, Zakharenko SS, Winton DJ, Zhu L, Gilbertson RJ. The NALCN channel regulates metastasis and nonmalignant cell dissemination. Nat Genet. 2022 Dec;54(12):1827-1838. doi: 10.1038/s41588-022-01182-0.
• Pagano E, Elias JE, Schneditz G, Saveljeva S, Holland LM, Borrelli F, Karlsen TH, Kaser A, Kaneider NC. Activation of the GPR35 pathway drives angiogenesis in the tumour microenvironment. Gut. 2022 Mar;71(3):509-520. doi: 10.1136/gutjnl-2020-323363.
• Saveljeva S, Sewell GW, Ramshorn K, Cader MZ, West JA, Clare S, Haag LM, de Almeida Rodrigues RP, Unger LW, Iglesias-Romero AB, Holland LM, Bourges C, Md-Ibrahim MN, Jones JO, Blumberg RS, Lee JC, Kaneider NC, Lawley TD, Bradley A, Dougan G, Kaser A. A purine metabolic checkpoint that prevents autoimmunity and autoinflammation. Cell Metab. 2022 Jan 4;34(1):106-124.e10. doi: 10.1016/j.cmet.2021.12.009.