Our research in the IMS-MRL focusses on understanding metabolism and metabolic disease affecting molecules, cells, tissues, organisms and populations. This ability to successfully undertake both “bench to bedside” and “bedside to bench” approaches helps us to make new discoveries, and investigate their significance.
Our staff group has a uniquely diverse skill set, with high proficiency at a wide range of laboratory and clinical research techniques. Our IMS-MRL League of Experts provides a resource for new staff members or existing staff members to collaborate or learn new techniques.
Scroll through the table below or use the search box to look for a particular person or technique.
|Name||Areas of Expertise|
Clinical Research Associate
|camb3 at medschl.cam.ac.uk||Medical education.
GLP1 and gut hormones.
PhD Student/Research Assistant
|kd464 at medschl.cam.ac.uk||In vitro cAMP assays.
|xd253 at medschl.cam.ac.uk||Western blotting.
Insulin tolerance test in mice.
Big dataset analysis.
|df220 at cam.ac.uk||Project management: Animal study design and modelling; Animal licencing and compliance; Data analysis interpretation.
Biochemistry: Western blotting; Immunoprecipitation; Immunohistochemistry; ELISA; Colorimetric assays.
Molecular biology: Isolation and analysis of DNA and RNA from cells and tissues; Design of primers and probes; PCR; qPCR
Chief Animal Technician (DMC)
|sef34 at medschl.cam.ac.uk||Mouse phenotyping (tail bleeds etc).|
Postdoctoral Research Associate
|ik352 at cam.ac.uk||Bioinformatics.
Non-Alcoholic Fatty Liver Disease.
|jk605 at medschl.cam.ac.uk||Clinical research.
Patient recruitment to clinical studies.
Post doctoral Research Associate
|lck34 at medschl.cam.ac.uk||Placental sampling.
Clinical study coordination.
Clinical study database design.
|Agnieszka (Agnes) Lukasik|
|al473 at medschl.cam.ac.uk||Public engagement.
Events and scientific conference organisation.
Promoting mental health and wellbeing.
Research Animal Technician
|mm2651 at medschl.cam.ac.uk||Mice handling.
Procedural work on mice.
Basic laboratory techniques in biology.
|clm70 at cam.ac.uk||Designing and running clinical studies.
Recruiting special populations.
Complex intervention studies.
pregnancy and childhood recruitment.
|Lucas Carminatti Pantaleao|
|lp435 at medschl.cam.ac.uk||Cell biology: extensive experience culturing eukaryotic cell lines and primary cells; generation of modified cell lines with siRNA and miRNA mimics using liposome transfection; generation and screening of stable KO cell lines using CRISPR-Cas9 plasmid vectors.
Cell based assays: transwell co-cultures; cell proliferation assays (e.g.: CFSE and MTT); Nile red staining of neutral lipids; Immunocytochemistry followed by fluorescent microscopy and FACS; radiolabelled fatty acid uptake and oxidation assays; SILAC.
Molecular biology: Isolation and analysis of DNA and RNA from cells and tissues; design of primers and probes; PCR; qPCR; DNA recombination and molecular cloning; plasmid engineering; DNA sequence analysis; RNA-seq; sRNA-Seq; cell-based luciferase assays.
Biochemistry: western blotting; immunoprecipitation; immunohistochemistry; ELISA; colorimetric assays.
Senior Research Associate
|cjp1002 at cam.ac.uk||Data analysis and interpretation.
General laboratory techniques in clinical biochemistry.
|kh271 at medschl.cam.ac.uk||Sanger sequencing.
Academic Clinical Fellow
|jt636 at cam.ac.uk||Single cell/nucleus RNA sequencing.
In situ hybridisation.
Histopathology (human and murine).
Clinical Research Midwife
|eht27 at medschl.cam.ac.uk||Recruitment of pregnant women onto a research study.|
Chief Research Technician
|jw939 at medschl.cam.ac.uk||Microtomy.
Staining (H&E and special stains).
In situ hybridisation (predominantly RNAscope).