The peptidomics and proteomics core offers mass spectrometric based support for researchers in the MRL-IMS, Cambridge University, wider academic institutes and industry partners. The laboratory uses a Thermo Q-Exactive Plus system with both nanoflow and normal flow rate LC separations for characterisation of peptides and proteins in biological matrices.

Bioinformatics analyses are performed using PEAKS, capable of performing normal proteomics experiments, and identifying endogenous peptides of up to 65 amino acids in length.

 

 

The facility is run by Dr Richard Kay (Gribble/Reimann group), who has over 15 years of experience in developing methods for quantifying peptides and proteins using LC-MS instrumentation. Prices for sample analysis are competitive and are available on request – email rgk27@medschl.cam.ac.uk

 

Examples of work carried out in the peptidomics and proteomics core:

 

Analysis of endogenous peptides in plasma, brain and intestinal tissue

Quantitative mass spectrometry for human melanocortin peptides in vitro and in vivo suggests prominent roles for β-MSH and desacetyl α-MSH in energy homeostasis, Molecular metabolism 17, 82-97, 2018

Gastrectomy with Roux-en-Y reconstruction as a lean model of bariatric surgery, Surgery for obesity and related diseases 14 (5), 562-568, 2018

Comparison of human and murine enteroendocrine cells by transcriptomic and peptidomic profiling, Diabetes 68 (5), 1062-1072, 2019

Peptidomic analysis of endogenous plasma peptides from patients with pancreatic neuroendocrine tumours, Rapid Communications in Mass Spectrometry 32 (16), 1414-1424, 2018

 

Absolute quantitation of peptides and proteins in biological matrices (Plasma, serum, tissue)

Assessment and Management of Anti-Insulin Autoantibodies in Varying Presentations of Insulin Autoimmune Syndrome, The Journal of Clinical Endocrinology & Metabolism 103 (10), 3845-3855 2018

Important role of the GLP-1 axis for glucose homeostasis after bariatric surgery, Cell reports 26 (6), 1399-1408, 2019

 

Assessment of peptide stability in biological matrices and identification of metabolites

Development and validation of an LC-MS/MS method for detection and quantification of in vivo derived metabolites of [Pyr1]apelin-13 in humans. Scientific Reports (in press)

 

Assessment of the control of secretion for peptides

Co-storage and release of insulin-like peptide-5, glucagon-like peptide-1 and peptideYY from murine and human colonic enteroendocrine cells. Molecular metabolism 16, 65-75, 2018

 

Videos:

LC-MS analysis of plasma from patients with insulin autoimmune syndrome

Analyzing intact Peptides using LC-MS for Translational Research