Brain metabolic sensing relies on the activity of specialized cells in discrete brain regions that can transduce hormonal and nutritional signals to drive endocrine, behavioural and autonomic output circuits regulating energy balance.
The Blouet Lab employs a multi-disciplinary approach coupling calcium imaging to characterize the neurophysiology of metabolic-sensing neurons, discrete manipulations of brain neurocircuits and nutrient sensing pathways using cutting-edge molecular genetics, and refined functional assessments in behaving rodents to characterize how nutrients are detected by the brain to maintain energy homeostasis in health and disease.
Heeley N, Kirwan P, Darwish T, Arnaud M, Evans ML, Merkle FT, Reimann F, Gribble FM, Blouet C. Rapid sensing of l-leucine by human and murine hypothalamic neurons: Neurochemical and mechanistic insights. Mol Metab. 2018 Feb 7. DOI:10.1016/j.molmet.2018.01.021. PMID: 29439854.
Burke LK, Darwish T, Cavanaugh AR, Virtue S, Roth E, Morro J, Liu SM, Xia J, Dalley JW, Burling K, Chua S, Vidal-Puig T, Schwartz GJ, Blouet C. mTORC1 in AGRP neurons integrates exteroceptive and interoceptive food-related cues in the modulation of adaptive energy expenditure in mice. Elife. 2017 May 23;6. DOI:10.7554/eLife.22848. PMID:28532548 PMCID:PMC5441868.
Cavanaugh AR, Schwartz GJ, Blouet C. High-fat feeding impairs nutrient sensing and gut brain integration in the caudomedial nucleus of the solitary tract in mice. PLoS One. 2015 Mar 16;10(3):e0118888. doi: 10.1371/journal.pone.0118888. PMID: 25774780. PMCID: PMC4361711
Blouet C, Jo YW, Li X, Schwartz GJ. Mediobasal hypothalamic leucine sensing regulates food intake through activation of a hypothalamus-brainstem circuit. J Neuroscience, 29(26):8302-11. 2009. PMID: 19571121. PMCID: PMC2740923.